文章摘要
引用本文:郑 磊,樊海平,吴 斌,曾占壮,钟全福,张新艳.罗非鱼无乳链球菌LAMP快速检测方法的建立[J].福州大学学报(自然科学版),2015,43(4):572~576
罗非鱼无乳链球菌LAMP快速检测方法的建立
Development of loop-mediated isothermal amplification for detection of Streptococcus agalactiae from Tilapia
  
DOI:10.7631/issn.1000-2243.2015.04.0572
中文关键词: 环介导等温扩增技术  罗非鱼  无乳链球菌  表面免疫相关蛋白基因  快速检测
英文关键词: LAMP  Tilapia  Streptococcus agalactiae  SIP  rapid detection
基金项目:
作者单位
郑 磊 福建省淡水水产研究所福建 福州 350002 
樊海平 福建省淡水水产研究所福建 福州 350002 
吴 斌 福建省淡水水产研究所福建 福州 350002 
曾占壮 福建省淡水水产研究所福建 福州 350002 
钟全福 福建省淡水水产研究所福建 福州 350002 
张新艳 福建省淡水水产研究所福建 福州 350002 
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中文摘要:
      针对无乳链球菌高度保守的表面免疫相关蛋白基因,采用环介导等温扩增技术(LAMP),设计4条特异性引物,优化反应体系,建立罗非鱼无乳链球菌快速诊断方法. 研究结果表明,LAMP方法检测灵敏度达到30 CFU·mL-1,且对2株罗非鱼源无乳链球菌均能扩增出特异性片段,而对14株非无乳链球菌均未扩增出相应片段. 建立的LAMP检测方法具有高度的特异性和灵敏性,反应在1 h内完成,为罗非鱼无乳链球菌病的快速诊断提供了一种重要的技术手段.
英文摘要:
      To develop a technique for rapid detection of Streptococcus agalactiae from Tilapia,the loop-mediated isothermal amplification(LAMP) was introduced to amplify the surface immunogenic protein(SIP) gene. The detection method was established by using optimum reaction system with four specific primers of the target gene. The results showed that the method had perfect specificity,and the sensitivity was 30 CFU·mL-1,as the DNA of 2 strains of Streptococcus agalactiae were able to be amplified,while the other 14 strains of non-Streptococcus agalactiae were not. The amplification process can be finished in 1 hour. The method provides a better choice for rapid detection of Streptococcus agalactiae.
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